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Sequence alignment between 2 Bacillus strains

Fig[1]: Sequence alignment of ggt gene in Bacillus licheniformis ATCC14580 and Bacillus licheniformis RK14-46. ggt gene of the RK14 and ATCC14580 strains differed only by two amino acid residues.

Fig[1]: Sequence alignment of ggt gene in Bacillus licheniformis ATCC14580 and Bacillus licheniformis RK14-46. ggt gene of the RK14 and ATCC14580 strains differed only by two amino acid residues.

The ggt gene sequence was selected in Bacillus paralicheniformis 9954a. A BLAST was run on this selected range. B. licheniformis ATCC14580 showed a 94.99% similarity. The genome sequence for RK14-46 was not available and thus , the accession number was not available.

Fig2: BLASTn result for B.licheniformis 14580 [2].

Fig2: BLASTn result for B.licheniformis 14580 [2].

B.licheniformis 14580 has a 99.89% similarity with B.licheniformis WX-02. Existing literature mentions the promoters used for over expression of the pgdS gene. We are yet to look into the promoters for inhibiting ggt gene expression. We hope to find similar promoter sequences from B.licheniformis WX-02 which we can use for B.licheniformis 14580.

Accession numbers

>CP000002.3:1354551-1356835 Bacillus licheniformis ATCC 14580, complete genome
>CP012110.1 Bacillus licheniformis WX-02 genome

Our Chosen Strain

Due to these reasons we chose Licheniformis ATCC 14580 as our strain. Plasmid for ggt gene deletion - pBGSC6

Fig3: Plasmid [3].

Fig3: Plasmid [3].

pBGSC6 is a simple, general-purpose integration vector for gram-positive organisms. An insert is cloned into the multiple cloning sites. The blue-white system on X-gal plates or red-white MacConkey agar detects the presence of the insert. The insert-bearing plasmid is used to transform the target organism with selection for chloramphenicol resistance. The plasmid integrates into the chromosome by homologous recombination[4].
Accession number of plasmid: DQ483056

References